分类
抗微生物试验 群落分析 异源表达系统 体内实验模型 微生物-宿主相互作用 微生物生物化学 微生物生物膜 微生物细胞生物学
微生物遗传学 CRISPR-Cas9
DNA 基因表达
基因图谱和克隆
基因组编辑
诱/突变
质粒
重组
RNA qRT-PCR
RNA 提取
测序
转录
转化
全基因组测序
微生物新陈代谢 微生物生理学 微生物蛋白质组学 微生物信号传导 病原体检测Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
外周血单核细胞中DNA总量及 2-LTR （长末端重复序列） HIV DNA、HIV RNA 和 疱疹病毒 DNA 的量化
Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including EBV) and this HIV reservoir (Gianella et al., 2014). This protocol was designed to simultaneously co-extract DNA and RNA from the same peripheral blood mononuclear cell (PBMC) aliquot and quantify HIV, CMV and EBV DNA, as well as HIV RNA using droplet digital PCR (ddPCR).
For collection and processing of male genital secretions and quantification of HIV RNA and DNA from seven human herpesviruses from seminal plasma, refer to protocol “Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma” (Vargas-Meneses et al., 2015).
For collection and processing of male genital secretions and quantification of HIV RNA and DNA from seven human herpesviruses from seminal plasma, refer to protocol “Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma” (Vargas-Meneses et al., 2015).